For my first three photographs I set my aperture to f/13 and then changed the ISO and shutter speed for each image. With a low ISO (eg 100) a slower shutter speed is required than at a high ISO setting (eg 1600). Each time the ISO is increased by one step it increases the exposer by one stop and therefore the shutter speed or aperture must be altered in order to obtain a correctly exposed photograph.
ISO 100, 1/25sec, f/13

ISO 400, 1/100sec, f/13

ISO 1600, 1/400sec, f/13

High ISO settings are great in low light situations, they allow a photographer to use faster shutter speeds to freeze movement, however the images suffer from a slight grainy effect. In some situations this could be an apealing feature but in others it can be an undesirable factor.
The grainy effect isn't very noticable unless the photographs are blown up to full resolution. I have cropped small similar sections from each image to see how noticable the grain is.
Smooth area
I feel that the grain is most noticable in dark areas. The darker fur in the first three crops show the distortion the most but the first two images are blurred and therefore I will compare two sharp images to see if the different is more or less noticable.ISO 100, 1/320sec, f/8

ISO 1600, 1/1250sec, f/8

Detailed view
Next I set my camera on a tripod to see if the grain is more noticable between identical photographs.
Below are five shots I have taken (one at each setting).
Click to enlarge
The difference between each of these shots is barely if at all noticable so I have cropped a small section from each to further display the difference between low and high ISO settings.
800, 1600
The difference between the lowest and highest setting is now more noticable. At the low end of the scale, the ISO 100 shot is crisp, clear and smooth whereas at 1600 the image is slightly grainy. The grain is much more noticable on dark areas. I feel that there is barely any difference between the shots and the effect would only be noticable if a photograph were to be scaled up to an A3 sized print. I need to experiment with film as I feel the grainy effect will be much more noticable. Having used ISO 400 film in the past I already know that to be quite grainy but I would like to see the difference between 100 and 800.